GFP anticorps (CypHer5E)

N° du produit ABIN1742297

1 publication est disponible pour ce produit. L’anticorps anti-GFP Polyclonal produit chez le Lapin a été validé pour ICC. Il convient pour la détection de GFP dans des échantillons provenant de Aequorea victoria. }

Aperçu rapide pour GFP anticorps (CypHer5E) (ABIN1742297)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la CypHer5E

Application: Immunocytochemistry (ICC)

Détail du produit anti-GFP anticorps (CypHer5E)

Fonction: Rabbit anti-GFP antibody

Attributs du produit: fluorescence-labeled with Cy pH er5E. Cy pH er5E® is a pH -sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. Cy pH er5E® can be detected with the filter sets for Cy5®. Cy pH er5E®: λex 647 nm / λem 667 nm

Purification: affinity purified

Information d'application

Indications d'application: WB:N/A IP:N/A ICC:1 : 100 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet

Commentaires:

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS

Agent conservateur: Without preservative

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C,-80 °C

Stockage commentaire: Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.

Références pour anticorps anti-GFP (CypHer5E) (ABIN1742297)

Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet:

Synonyms: Sirius, meGFP, eGFP, paGFP, acGFP, eYFP, sfGFP, Green fluorescent protein, supereclyptic pH luorin, Cerulean, eCFP, mTFP, Venus, Citrine

Antigen Information: GFP is a widely used green fluorescent protein tag