RIMS2 anticorps (AA 461-987)

N° du produit ABIN1742308

Cet anticorps Lapin Polyclonal détecte spécifiquement RIMS2 dans WB, IHC (p) et ICC. Il présente une réactivité avec des échantillons de Rat, Souris, Poulet, Hamster et Poisson zèbre (Danio rerio). Il a été cité dans 13+ publications.

Aperçu rapide pour RIMS2 anticorps (AA 461-987) (ABIN1742308)

Antigène: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Reactivité: Rat, Souris, Poulet, Hamster, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RIMS2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-RIMS2 anticorps

Épitope: AA 461-987

Fonction: anti-RIM2

Specificité: RIM 2 including splice variants

Réactivité croisée (Details): Reacts with: rat (Q9JIS1), mouse (Q9EQZ7), hamster, chicken, zebrafish. Other species not tested yet.

Purification: affinity purified

Immunogène: Recombinant protein corresponding to AA 461 to 987 from rat Rim2 (UniProt Id: Q9JIS1)

Information d'application

Indications d'application: WB:1 : 100 up to 1 : 1000 , IHC-P:1 : 500,

Commentaires:

This antibody works with paraformaldehyde and methanol fixation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C,-80 °C,4 °C

Stockage commentaire: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Références pour anticorps anti-RIMS2 (ABIN1742308)

Mendoza Schulz, Jing, Sánchez Caro, Wetzel, Dresbach, Strenzke, Wichmann, Moser: "Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." dans: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, (2014) (PubMed).

de Andrade, Kunzelman, Merrill, Fuerst: "Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina." dans: Molecular vision, Vol. 20, pp. 1422-33, (2014) (PubMed).

Gardezi, Li, Stanley: "Inter-channel scaffolding of presynaptic CaV2.2 via the C terminal PDZ ligand domain." dans: Biology open, Vol. 2, Issue 5, pp. 492-8, (2013) (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Gebhart, Juhasz-Vedres, Zuccotti, Brandt, Engel, Trockenbacher, Kaur, Obermair, Knipper, Koschak, Striessnig: "Modulation of Cav1.3 Ca2+ channel gating by Rab3 interacting molecule." dans: Molecular and cellular neurosciences, Vol. 44, Issue 3, pp. 246-59, (2010) (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandstätter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." dans: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).

Kwan, Xie, Sheu, Ohtsuka, Gaisano: "Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells." dans: Diabetes, Vol. 56, Issue 10, pp. 2579-88, (2007) (PubMed).

Khanna, Zougman, Stanley: "A proteomic screen for presynaptic terminal N-type calcium channel (CaV2.2) binding partners." dans: Journal of biochemistry and molecular biology, Vol. 40, Issue 3, pp. 302-14, (2007) (PubMed).

Inoue, Deguchi-Tawarada, Takao-Rikitsu, Inoue, Kitajima, Ohtsuka, Takai: "ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 6, pp. 659-72, (2006) (PubMed).

Juranek, Mukherjee, Rickmann, Martens, Calka, Südhof, Jahn: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." dans: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, (2006) (PubMed).

tom Dieck, Altrock, Kessels, Qualmann, Regus, Brauner, Fejtová, Bracko, Gundelfinger, Brandstätter: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." dans: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, (2005) (PubMed).

Ohara-Imaizumi, Ohtsuka, Matsushima, Akimoto, Nishiwaki, Nakamichi, Kikuta, Nagai, Kawakami, Watanabe, Nagamatsu et al.: "ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic ..." dans: Molecular biology of the cell, Vol. 16, Issue 7, pp. 3289-300, (2005) (PubMed).

Détails sur RIMS2

Antigène: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Autre désignation: RIM2

Sujet: Active Zone, CAZ

UniProt: Q9JIS1

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis