Bassoon anticorps (C-Term)

N° du produit ABIN1742310

Détail du produit anti-Bassoon anticorps

Antigène: Bassoon (BSN)

Épitope: C-Term

Reactivité: Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bassoon est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificité: Specific for bassoon.

Purification: hybridoma supernatant

Immunogène: Recombinant protein containing the 330 C-terminal aa of rat bassoon.

Clone: 219E1

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

Commentaires:

ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-Bassoon (ABIN1742310)

Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." dans: eLife, Vol. 5, (2016) (PubMed).

Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." dans: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Schröder, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." dans: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).

Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).

Détails sur Bassoon

Antigène: Bassoon (BSN)

Autre désignation: Bassoon (BSN Produits)