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Synaptojanin 1 anticorps (Isoform 2)

Cet anticorps anti-Synaptojanin 1 est un anticorps Lapin Polyclonal détectant Synaptojanin 1 dans WB et ICC. Adapté pour Rat, Souris et Hamster. Ce Primary Antibody a été cité dans 7+ publications.
N° du produit ABIN1742316

Aperçu rapide pour Synaptojanin 1 anticorps (Isoform 2) (ABIN1742316)

Antigène

Voir toutes Synaptojanin 1 (SYNJ1) Anticorps
Synaptojanin 1 (SYNJ1)

Reactivité

  • 22
  • 18
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Rat, Souris, Hamster

Hôte

  • 31
  • 6
Lapin

Clonalité

  • 32
  • 5
Polyclonal

Conjugué

  • 19
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Synaptojanin 1 est non-conjugé

Application

  • 18
  • 13
  • 13
  • 13
  • 5
  • 4
  • 3
  • 2
  • 2
Western Blotting (WB), Immunocytochemistry (ICC)
  • Épitope

    • 15
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1292-1308, Isoform 2

    Specificité

    Recognizes all isoforms of synaptojanin 1.

    Purification

    Affinity purified with the immunogen.

    Immunogène

    Synthetic peptide CLPSDSSPQLQQEQP (aa 1292-1308 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
  • Indications d'application

    WB: 1:200 up to 1:2000 ,

    Commentaires

    Running SDS-PAGE with non-boiled samples gives stronger signals.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 50µl H2O to get a 1mg/ml solution in PBS.

    Buffer

    PBS. Albumin and azide were added for stabilization.

    Agent conservateur

    Sodium azide

    Conseil sur la manipulation

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    Stock

    -20 °C

    Stockage commentaire

    Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.
  • Cliburn, Dunn, Stout, Hoffman, Lohr, Bernstein, Winokur, Burkett, Schmitz, Caudle, Miller: "Immunochemical localization of vesicular monoamine transporter 2 (VMAT2) in mouse brain." dans: Journal of chemical neuroanatomy, (2016) (PubMed).

    Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." dans: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Park, Kim, Lee, Park, Park, Sun, Kim, Chang: "SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane." dans: Journal of cell science, Vol. 123, Issue Pt 10, pp. 1742-50, (2010) (PubMed).

    Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

    Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

    Shin, Lee, Ahn, Kim, Ahn, YongPark, Chang: "Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis." dans: The Journal of biological chemistry, Vol. 282, Issue 39, pp. 28939-50, (2007) (PubMed).

  • Antigène

    Synaptojanin 1 (SYNJ1)

    Autre désignation

    Synaptojanin 1

    Pathways

    Inositol Metabolic Process, Synaptic Vesicle Exocytosis
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