Ribeye (AA 974-988) anticorps

N° du produit ABIN1742396

Détail du produit

Antigène: Ribeye

Épitope: AA 974-988

Reactivité: Souris, Rat, Boeuf (Vache), Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: This antibody recognizes ribeye and CtBP 2 (cat. no. 193 003).

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Synthetic peptide corresponding to aa 974-988 of rat ribeye coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 10000

Commentaires:

The immunogen is identical to that of anti-CtBP 2 (ABIN1742398).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Référence

Zabouri, Haverkamp: "Calcium channel-dependent molecular maturation of photoreceptor synapses." dans: PLoS ONE, Vol. 8, Issue 5, pp. e63853, (2013) (PubMed).

Müller, Butz, Peichl, Haverkamp: "The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified AII amacrine cells." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1014-23, (2013) (PubMed).

Hübler, Rankovic, Richter, Lazarevic, Altrock, Fischer, Gundelfinger, Fejtova: "Differential spatial expression and subcellular localization of CtBP family members in rodent brain." dans: PLoS ONE, Vol. 7, Issue 6, pp. e39710, (2012) (PubMed).

Détail du antigène

Antigène: Ribeye