GABRA1 anticorps (N-Term)

N° du produit ABIN1742457

Cet anticorps anti-GABRA1 est un anticorps Cobaye Polyclonal détectant GABRA1 dans WB et ICC. Adapté pour Rat et Souris.

Aperçu rapide pour GABRA1 anticorps (N-Term) (ABIN1742457)

Antigène: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivité: Rat, Souris

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp GABRA1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-GABRA1 anticorps

Épitope: N-Term

Fonction: anti-GABA-A receptor alpha1

Specificité: Specific for GABA-A receptor alpha1.

Réactivité croisée (Details): Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.

Purification: affinity purified

Immunogène: Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor alpha1. (UniProt Id: P62813)

Information d'application

Indications d'application: WB:1 : 1000 up to 1 : 2000 , IHC-P:not tested yet

Commentaires:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C,-80 °C,4 °C

Stockage commentaire: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Détails sur GABRA1

Antigène: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Autre désignation: GABA-A receptor alpha1

Sujet: GABA receptor, Gabra1

UniProt: P62813