beta Actin anticorps (Actin, beta) (AA 2-16)

Details for Product anti-ACTB Antibody No. ABIN1742508, Fournisseur: Connectez-vous pour afficher
Antigène
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Épitope
AA 2-16
159
37
17
17
10
8
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Poulet, Souris, Rat (Rattus), Poisson zèbre (Danio rerio)
595
356
309
109
87
84
68
58
54
44
42
41
32
32
27
23
16
16
15
15
9
9
8
8
7
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Hôte
Lapin
367
253
14
7
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp beta Actin est non-conjugé
36
31
19
6
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
582
253
180
141
110
100
58
50
37
18
14
13
12
9
8
2
2
2
2
2
1
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
'Independent Validation' signe
Numéro du lot 251003/3
Application validée Western Blotting
Contrôle positif mouse brain, mouse kidney, mouse muscle, mouse spleen, mouse testes
Contrôle négative none [ubiquitously expressed]
Conclusion A band was observed in the positive controls at the expected size (~42 kDa)
Anticorps primaire
Anticorps secondaire
Contrôles
  • All lysates used were prepared by Kinexus Bioinformatics Corporation following standard protocols and quality controlled for protein integrity on a regular basis.
  • Loading control: blot was stripped and re-probed with β3-Tubulin.
Protocole
  • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
  • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
  • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
  • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
  • 6. The membrane was washed in TTBS twice for 5 minutes each.
  • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
  • 8. The membrane was rinsed twice with TTBS.
  • 9. The membrane was washed in TTBS twice for 5 minutes each.
  • 10. The membrane was washed in TTBS once for 15 minutes.
  • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
  • 12. The membrane was rinsed twice with TTBS.
  • 13. The membrane was washed in TTBS twice for 5 minutes each.
  • 14. The membrane was washed in TTBS once for 15 minutes.
  • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 160 seconds.
  • 16. The membrane was rinsed three times with TTBS.
  • 17. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 18. The membrane was washed in TTBS 3 times for 5 minutes each.
  • 19. Repeated Steps 4-14 with the loading control antibody (for β3−Tubulin).
  • 20. Repeated Steps 4-14 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
Notes A protein band was observed at the expected target size of β−Actin of 42 kDa in all mouse extracts tested. Lower molecular weight bands of approximately 28-30 kDa were also observed in the mouse kidney and spleen tissue samples. Three main groups of actin have been identified. The β-actins and γ-actins co-exist in most cell types as components of the cytoskeleton, whereas the α-actin are found in muscle tissues. In our experience, we find that the level of actin does vary widely between different cell and tissue types.
Validation Images
Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) Figure 1. Western blot for beta actin (ACTB). Grey arrowhead indicates the expected m...
Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) Figure 2. Ponceau stain loading control.
Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) Figure 3. Western blot for beta actin (ACTB) using attempted siRNA silencing. Arrowhe...
Immunogène Synthetic peptide (aa 2-16 of mouse beta-actin) coupled to KLH via a C-terminally added cysteine.
Réactivité croisée (Details) May cross-react to alpha- and gamma-actin due to sequence homology.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Plasmids, Primers & others Plasmids, Primers & others beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation beta-Actin (ACTB Antibody Extrait)
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Indications d'application WB: 1 : 1000 up to 1 : 5000
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 200
Commentaires

ICC: Methanol fixation is recommended.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Conseil sur la manipulation Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock -20 °C
Stockage commentaire Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Images (Fournisseur)
Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) anti-Actin, beta (ACTB) (AA 2-16) antibody
Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) anti-Actin, beta (ACTB) (AA 2-16) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) anti-Actin, beta (ACTB) (AA 2-16) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508) Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle sec...
Produit citée dans: Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." dans: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, 2013 (PubMed).

Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, 2012 (PubMed).

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