Glu-alpha-Tubulin (C-Term) anticorps

N° du produit ABIN1742545

Cet anticorps Souris Monoclonal détecte spécifiquement dans ELISA, IHC, WB et ICC. Il présente une réactivité envers Humain, Souris, Rat, Eucaryotes et Poisson zèbre (Danio rerio) et a été mentionné dans 9+ publications.

Aperçu rapide pour Glu-alpha-Tubulin (C-Term) anticorps (ABIN1742545)

Antigène: Glu-alpha-Tubulin

Reactivité: Humain, Souris, Rat, Eucaryotes, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 1D5

Détail du produit

Épitope: C-Term

Specificité: Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).

Réactivité croisée (Details): No cross reaction to tyrosinated tubulin.

Immunogène: Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:1000, ICC: 1:200 up to 1:500, IHC: 1:500, IHC-P: 1:1000,

Commentaires:

WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C

Stockage commentaire: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

Référence

Zink, Grosse, Freikamp, Bänfer, Müksch, Jacob: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." dans: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).

Jablonka, Dombert, Asan, Sendtner: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." dans: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).

Roll-Mecak, Vale: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." dans: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).

Zhang, Gilquin, Khochbin, Matthias: "Two catalytic domains are required for protein deacetylation." dans: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).

Rosales-Nieves, Johndrow, Keller, Magie, Pinto-Santini, Parkhurst: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." dans: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).

Yoon, Shin, Mercurio: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." dans: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).

Van der Heiden, Groenendijk, Hierck, Hogers, Koerten, Mommaas, Gittenberger-de Groot, Poelmann: "Monocilia on chicken embryonic endocardium in low shear stress areas." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).

Rüdiger, Rüdiger, Wehland, Weber: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." dans: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).

Wheatley, Feilen, Yin, Wheatley: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." dans: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).

Détail du antigène

Antigène: Glu-alpha-Tubulin