Thrombospondin 1 anticorps (N-Term)

N° du produit ABIN1781811

Cet anticorps anti-Thrombospondin 1 est un anticorps Chèvre Polyclonal détectant Thrombospondin 1 dans WB, IHC, ELISA, IF et FACS. Adapté pour Humain et Souris.

Aperçu rapide pour Thrombospondin 1 anticorps (N-Term) (ABIN1781811)

Antigène: Thrombospondin 1 (THBS1)

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Thrombospondin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-Thrombospondin 1 anticorps

Épitope: N-Term

Fonction: Thrombospondin

Séquence: NRIPESGGDN SVFD

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Mouse Kidney lysates (calculated MW of 129 kDa according to NP_035710.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Thrombospondin 1

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: THBS1

Sujet: THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180

ID gène: 7057

NCBI Accession: NP_003237

Pathways: Autophagy