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Follistatin anticorps

L’anticorps Lapin Polyclonal anti-Follistatin a été validé pour WB et EIA. Il convient pour détecter Follistatin dans des échantillons de Humain.
N° du produit ABIN181535

Aperçu rapide pour Follistatin anticorps (ABIN181535)

Antigène

Voir toutes Follistatin (FST) Anticorps
Follistatin (FST)

Reactivité

  • 54
  • 19
  • 17
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
Humain

Hôte

  • 38
  • 18
  • 2
Lapin

Clonalité

  • 42
  • 16
Polyclonal

Conjugué

  • 45
  • 6
  • 3
  • 2
  • 1
  • 1
Cet anticorp Follistatin est non-conjugé

Application

  • 46
  • 31
  • 22
  • 8
  • 7
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Specificité

    This antibody detetcs Follistatin.

    Purification

    Affinity purified

    Immunogène

    Highly pure (> 98 %) recombinant human Follistatin
  • Indications d'application

    ELISA: Direct: To detect hFollistatin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanFollistatin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

    Buffer

    PBS, pH 7.2

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    Date de péremption

    6 months
  • Antigène

    Follistatin (FST)

    Autre désignation

    Follistatin

    Sujet

    Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing.Synonyms: Activin-binding protein, FS, FST

    ID gène

    10468

    NCBI Accession

    NP_006341

    UniProt

    P19883

    Pathways

    Negative Regulation of Hormone Secretion
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