Interleukin 17a anticorps (Biotin)

N° du produit ABIN181569

Cet anticorps anti-Interleukin 17a est un anticorps Chèvre Polyclonal détectant Interleukin 17a dans WB et EIA. Adapté pour Humain.

Aperçu rapide pour Interleukin 17a anticorps (Biotin) (ABIN181569)

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Interleukin 17a est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Interleukin 17a anticorps (Biotin)

Specificité: This antibody detects Interleukin-17A.

Purification: Affinity chromatography

Immunogène: Highly pure (> 98 %) recombinant human IL-17A

Information d'application

Indications d'application: ELISA: Direct: To detect hIL-17A by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Sandwich: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanIL-17A as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur Interleukin 17a

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: Interleukin-17A (IL17A)

Sujet: IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17

ID gène: 3605

NCBI Accession: NP_002181

UniProt: Q16552