IL-19 anticorps (Biotin)

N° du produit ABIN181613

L’anticorps anti-IL-19 Polyclonal Lapin est utilisé pour la détection de IL-19 dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour IL-19 anticorps (Biotin) (ABIN181613)

Antigène: IL-19 (IL19) (Interleukin 19 (IL19))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-19 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-19 anticorps (Biotin)

Specificité: This antibody detects Interleukin-19.

Purification: Affinity chromatography

Immunogène: Highly pure (> 98 %) recombinant human IL-19

Information d'application

Indications d'application: ELISA: Direct: To detect hIL-19 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Sandwich: To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human IL-19 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hIL-19. Western Blot: To detect hIL-19 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur IL-19

Antigène: IL-19 (IL19) (Interleukin 19 (IL19))

Autre désignation: Interleukin-19 / IL19

Sujet: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: IL-19, Melanoma differentiation-associated protein-like protein, NG.1, ZMDA

ID gène: 29949

NCBI Accession: NP_037503

UniProt: Q9UHD0