CCL4 anticorps (Biotin)

N° du produit ABIN181625

L’anticorps Lapin Polyclonal anti-CCL4 a été validé pour WB et EIA. Il convient pour détecter CCL4 dans des échantillons de Souris.

Aperçu rapide pour CCL4 anticorps (Biotin) (ABIN181625)

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CCL4 anticorps (Biotin)

Specificité: This antibody detects Macrophage Inflammatory Protein-1 beta.

Purification: Affinity chromatography

Immunogène: Highly pure (> 98 %) recombinant murine MIP-1beta

Information d'application

Indications d'application: ELISA: Direct: To detect mMIP-1β by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mMIP-1β. Sandwich: To detect mMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine MIP-1β as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mMIP-1β. Western Blot: To detect mMIP-1β by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMIP-1β is 1.5 - 3.0 ng/lane, under either reducing or

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur CCL4

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Autre désignation: MIP1 beta / CCL4

Sujet: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

ID gène: 20303

NCBI Accession: NP_038680

UniProt: P14097