MIA anticorps (Biotin)

N° du produit ABIN181669

Détail du produit anti-MIA anticorps (Biotin)

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIA est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody detects MIA.

Purification: Affinity Chromatography.

Immunogène: Highly pure (> 98%) recombinant Human MIA

Information d'application

Indications d'application: Direct ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentration ofapprox. 1.0 μg/mL of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. In conjunction with PolyclonalAnti-Human MIA (ABIN181672 or ABIN181671) as a Capture antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur MIA

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA (MIA Produits)

Synonymes: anticorps CD-RAP, anticorps Mia1, anticorps Cdrap, anticorps zgc:194157, anticorps mia, anticorps CDRAP, anticorps Fdp, anticorps MIA, anticorps MIAL, anticorps melanoma inhibitory activity, anticorps otoraplin, anticorps cilia and flagella associated protein 100, anticorps MIA, anticorps Mia, anticorps mia, anticorps Otor, anticorps CFAP100

Sujet: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

ID gène: 8190

NCBI Accession: NP_001189482

UniProt: Q16674