MIA anticorps

N° du produit ABIN181672

Cet anticorps anti-MIA est un anticorps Lapin Polyclonal détectant MIA dans WB, IHC (p) et EIA. Adapté pour Humain.

Aperçu rapide pour MIA anticorps (ABIN181672)

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-MIA anticorps

Specificité: This antibody detects MIA.

Purification: Immunoaffinity Chromatography.

Immunogène: Highly pure (> 98%) recombinant Human MIA

Information d'application

Indications d'application: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sectionsof human pancreas infiltrating ductal adenocarcinoma tissue. The recommendedconcentration is 1.0-2.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeledpolymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimalresults for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at roomtemperature.
Optimal conditions may vary. Tissue samples were provided by theCooperative Human Tissue Network which is funded by the National Cancer Institute. Indirect ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human MIA (ABIN181670 or ABIN181669) as a Detection antibody, itallows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur MIA

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA

Sujet: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

ID gène: 8190

NCBI Accession: NP_001189482

UniProt: Q16674