IL-31 anticorps

N° du produit ABIN181696

Cet anticorps anti-IL-31 Polyclonal Lapin (ABIN181696) détecte spécifiquement IL-31 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour IL-31 anticorps (ABIN181696)

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-31 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-31 anticorps

Specificité: This antibody detects Human Interleukin-31.

Purification: Immunoaffinity Chromatography

Immunogène: Highly pure (> 98%) E.coli derived recombinant Human IL-31

Information d'application

Indications d'application: ELISA: Indirect: To detect hIL-31 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31. Sandwich: To detect hIL-31 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIL-31 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-31. Western blot: To detect hIL-31 this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution the antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur IL-31

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Autre désignation: Interleukin-31 / IL31

Sujet: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia and skin lesions, and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.Synonyms: IL-31

ID gène: 386653

NCBI Accession: NP_001014358

UniProt: Q6EBC2