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beta Defensin 1 anticorps

L’anticorps Lapin Polyclonal anti-beta Defensin 1 a été validé pour WB et EIA. Il convient pour détecter beta Defensin 1 dans des échantillons de Humain.
N° du produit ABIN181704

Aperçu rapide pour beta Defensin 1 anticorps (ABIN181704)

Antigène

Voir toutes beta Defensin 1 (DEFB1) Anticorps
beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivité

  • 48
  • 24
  • 9
  • 8
  • 5
Humain

Hôte

  • 63
  • 13
  • 2
  • 1
Lapin

Clonalité

  • 64
  • 15
Polyclonal

Conjugué

  • 38
  • 13
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp beta Defensin 1 est non-conjugé

Application

  • 52
  • 31
  • 23
  • 19
  • 19
  • 13
  • 13
  • 7
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Specificité

    This antibody detects Defensin beta 1.

    Purification

    Immunoaffinity chromatography

    Immunogène

    Highly pure (> 98 %) recombinant human BD-1
  • Indications d'application

    ELISA: Indirect: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. This In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Sandwich: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanBD-1 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBD-1. Western blot: To detect hBD-1 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

    Buffer

    PBS, pH 7.2

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    Date de péremption

    6 months
  • Antigène

    beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

    Autre désignation

    Defensin beta 1

    Sujet

    Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

    ID gène

    1672

    NCBI Accession

    NP_005209

    UniProt

    P60022
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