beta Defensin 1 anticorps

N° du produit ABIN181704

L’anticorps anti-beta Defensin 1 Polyclonal Lapin est utilisé pour la détection de beta Defensin 1 dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour beta Defensin 1 anticorps (ABIN181704)

Antigène: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Defensin 1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-beta Defensin 1 anticorps

Specificité: This antibody detects Defensin beta 1.

Purification: Immunoaffinity chromatography

Immunogène: Highly pure (> 98 %) recombinant human BD-1

Information d'application

Indications d'application: ELISA: Indirect: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. This In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Sandwich: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanBD-1 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBD-1. Western blot: To detect hBD-1 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur beta Defensin 1

Antigène: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Autre désignation: Defensin beta 1

Sujet: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

ID gène: 1672

NCBI Accession: NP_005209

UniProt: P60022