Thymic Stromal Lymphopoietin anticorps

N° du produit ABIN181720

Détail du produit anti-Thymic Stromal Lymphopoietin anticorps

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Thymic Stromal Lymphopoietin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects HumanTSLP. Other species not tested.

Purification: Immunoaffinity Chromatography

Immunogène: Highly pure (> 98%) E.coli derived recombinant Human TSLP

Information d'application

Indications d'application: ELISA: Indirect: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. Sandwich: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanTSLP as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTSLP. Western blot: To detect hTSLP analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur Thymic Stromal Lymphopoietin

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Autre désignation: TSLP (TSLP Produits)

Synonymes: anticorps TSLP, anticorps thymic stromal lymphopoietin, anticorps TSLP, anticorps Tslp

Sujet: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.Synonyms: Thymic stroma-derived lymphopoietin, Thymic stromal lymphopoietin

ID gène: 53603

NCBI Accession: NP_067342

UniProt: Q9JIE6