His Tag anticorps (FITC)

N° du produit ABIN181860

Cet anticorps anti-His Tag Monoclonal Souris conjugué à FITC (Clone AD1-1-10) (ABIN181860) détecte spécifiquement His Tag dans IF et FACS.

Aperçu rapide pour His Tag anticorps (FITC) (ABIN181860)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AD1-1-10

Détail du produit anti-His Tag anticorps (FITC)

Séquence: HHHHHH

Purification: Affinity Chromatography on Protein G

Immunogène: PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.