MHC, Class I anticorps (FITC)

N° du produit ABIN181880

L’anticorps anti-MHC, Class I Monoclonal Souris est utilisé pour la détection de MHC, Class I dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Cobaye, Porc et Hamster. Il a été validé pour FACS.

Aperçu rapide pour MHC, Class I anticorps (FITC) (ABIN181880)

Antigène: MHC, Class I

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Porc, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC, Class I est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: 2G5

Détail du produit anti-MHC, Class I anticorps (FITC)

Purification: Affinity chromatography on Protein G

Immunogène: Purified H-2Kb and H-2Db MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MHC, Class I

Antigène: MHC, Class I

Autre désignation: MHC Class I

Sujet: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I