SIRPA anticorps

N° du produit ABIN182013

Cet anticorps anti-SIRPA est un anticorps Souris Monoclonal détectant SIRPA dans WB, FACS, IHC (p) et IHC (fro). Adapté pour Rat.

Aperçu rapide pour SIRPA anticorps (ABIN182013)

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SIRPA est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-41

Détail du produit anti-SIRPA anticorps

Specificité: This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

Purification: Affinity chromatography on Protein G

Immunogène: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires protein digestion pre- treatment of paraffin sections e.g. trypsin or pronase. Flow Cytometry: 1/100 - 1/200, Use 10 mLof the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SIRPA

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Autre désignation: CD172a / SIRPA

Sujet: Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

ID gène: 25528

NCBI Accession: NP_037148

UniProt: P97710