CD5 anticorps (FITC)

N° du produit ABIN182022

Cet anticorps anti-CD5 est un anticorps Rat Monoclonal détectant CD5 dans FACS. Adapté pour Chien.

Aperçu rapide pour CD5 anticorps (FITC) (ABIN182022)

Antigène: CD5

Reactivité: Chien

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: YKIX322-3

Détail du produit anti-CD5 anticorps (FITC)

Purification: Affinity chromatography on Protein G

Immunogène: Concanavilin A activated canine peripheral blood cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: CD5 is a 55 kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.Synonyms: LEU-1, LEU1, Ly-1, Lymphocyte antigen T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5