CBP Tag anticorps

N° du produit ABIN1842586

L’anticorps Souris Monoclonal anti-CBP Tag a été validé pour WB et ELISA. Il convient pour détecter CBP Tag dans des échantillons de . Il y a 1 publication disponible.

Aperçu rapide pour CBP Tag anticorps (ABIN1842586)

Antigène: CBP Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CBP Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 1D11E5

Détail du produit anti-CBP Tag anticorps

Séquence: KRRWKKNFIA VSAA

Specificité: CBP Tag Antibody, mAb, Mouse recognizes CBP-tagged proteins.

Attributs du produit: CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.

Purification: Protein A affinity column

Immunogène: CBP tag peptide conjugated to KLH

Isotype: IgG2b kappa

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western blot: 1 µg/mL.
Immunoprecipitation: 1-5 µg/mL.
ELISA: 0. 001-0. 002 µg/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Références pour anticorps anti-CBP Tag (ABIN1842586)

Li, Liu, Zhang, Issaian, Hill, Espinosa, Shi, Cui, Kappel, Das, Hansen, Zhou, Zhao: "A unified mechanism for intron and exon definition and back-splicing." dans: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).

Détails sur CBP Tag

Antigène: CBP Tag

Classe de substances: Tag

Sujet: CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins