BCL7A anticorps (C-Term)

N° du produit ABIN184562

L’anticorps Chèvre Polyclonal anti-BCL7A a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter BCL7A dans des échantillons de Humain.

Aperçu rapide pour BCL7A anticorps (C-Term) (ABIN184562)

Antigène: BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BCL7A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-BCL7A anticorps

Épitope: C-Term

Fonction: BCL7A

Séquence: MKLEASQQNS EEM

Specificité: This antibody is expected to recognize both reported isoforms (NP_066273.1, NP_001019979.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-MKLEASQQNSEEM, from the C Terminus of the protein sequence according to NP_066273.1, NP_001019979.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 26 kDa band observed in Human Lymph Node lysates (calculated MW of 25.0 kDa according to NP_066273.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur BCL7A

Antigène: BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

Autre désignation: BCL7A

Sujet: BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7

ID gène: 605, 77045

NCBI Accession: NP_066273, NP_001019979