FOXP3 anticorps (C-Term)

N° du produit ABIN184677

Détail du produit anti-FOXP3 anticorps

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXP3 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Fonction: FOXP3 / SCURFIN

Séquence: SQRPSRCSNP TPGP

Specificité: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur FOXP3

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Autre désignation: FOXP3 (FOXP3 Produits)

Synonymes: anticorps foxp3, anticorps FOXP3, anticorps AIID, anticorps DIETER, anticorps IPEX, anticorps PIDX, anticorps XPID, anticorps JM2, anticorps scurfin, anticorps sf, anticorps RGD1562112, anticorps forkhead box P3, anticorps forkhead box P3a, anticorps forkhead box P3 L homeolog, anticorps FOXP3, anticorps foxp3a, anticorps foxp3, anticorps foxp3.L, anticorps Foxp3

Sujet: FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin

ID gène: 50943

NCBI Accession: NP_054728, NP_001107849

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation