NUMB anticorps (C-Term)

N° du produit ABIN184679

L’anticorps Chèvre Polyclonal anti-NUMB a été validé pour IHC, ELISA, IF et FACS. Il convient pour détecter NUMB dans des échantillons de Humain et Souris.

Aperçu rapide pour NUMB anticorps (C-Term) (ABIN184679)

Antigène: NUMB (Numb Homolog (NUMB))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NUMB est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-NUMB anticorps

Épitope: C-Term

Fonction: NUMB

Séquence: PFSSDLQKTF EIEL

Specificité: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet b

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 6-8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NUMB

Antigène: NUMB (Numb Homolog (NUMB))

Autre désignation: NUMB

Sujet: NUMB, numb homolog (Drosophila), S171, numb homolog

ID gène: 8650, 18222

NCBI Accession: NP_001005743, NP_003735, NP_001005745

Pathways: Cell-Cell Junction Organization