ELMO2 anticorps (C-Term)

N° du produit ABIN184684

Cet anticorps Chèvre Polyclonal détecte spécifiquement ELMO2 dans WB, ELISA, IHC, FACS et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour ELMO2 anticorps (C-Term) (ABIN184684)

Antigène: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ELMO2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ELMO2 anticorps

Épitope: C-Term

Fonction: ELMO2

Séquence: PKEPSSYDFV YHYG

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-PKEPSSYDFVYHYG

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6 kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7 kDa according

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ELMO2

Antigène: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Autre désignation: ELMO2

Sujet: ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2

ID gène: 63916, 140579

NCBI Accession: NP_877496, NP_573403