ELMO2 anticorps (C-Term)

N° du produit ABIN184684

Détail du produit anti-ELMO2 anticorps

Antigène: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ELMO2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: ELMO2

Séquence: PKEPSSYDFV YHYG

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: C-PKEPSSYDFVYHYG

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6 kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7 kDa according

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ELMO2

Antigène: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Autre désignation: ELMO2 (ELMO2 Produits)

Synonymes: anticorps CED-12, anticorps CED12, anticorps ELMO-2, anticorps ELMO2, anticorps MGC114811, anticorps 1190002F24Rik, anticorps mKIAA1834, anticorps engulfment and cell motility 2, anticorps engulfment and cell motility protein 2, anticorps engulfment and cell motility 2 L homeolog, anticorps ELMO2, anticorps LOC100232489, anticorps Elmo2, anticorps elmo2.L, anticorps elmo2

Sujet: ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2

ID gène: 63916, 140579

NCBI Accession: NP_877496, NP_573403