KLF3 anticorps (N-Term)

N° du produit ABIN184727

L’anticorps Chèvre Polyclonal anti-KLF3 a été validé pour WB, IF, ELISA et FACS. Il convient pour détecter KLF3 dans des échantillons de Humain.

Aperçu rapide pour KLF3 anticorps (N-Term) (ABIN184727)

Antigène: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KLF3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-KLF3 anticorps

Épitope: N-Term

Fonction: KLF3 / BKLF

Séquence: LMFDPVPVKQ EAMD

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence LMFDPVPVKQEAMD-C, from the N Terminus of the protein sequence according to NP_057615.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 38-40 kDa band observed in Human Heart lysates (predicted MW of 38.8Da according to NP_057615.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur KLF3

Antigène: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Autre désignation: KLF3

Sujet: KLF3, BKLF, Kruppel-like factor 3 (basic), basic kruppel like factor, MGC48279

ID gène: 51274

NCBI Accession: NP_057615