Flotillin 1 anticorps (C-Term)

N° du produit ABIN184756

Cet anticorps Chèvre Polyclonal détecte spécifiquement Flotillin 1 dans WB, ELISA et IHC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Flotillin 1 anticorps (C-Term) (ABIN184756)

Antigène: Flotillin 1 (FLOT1)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Flotillin 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-Flotillin 1 anticorps

Épitope: C-Term

Fonction: Flotillin 1

Séquence: SISQVNHKPL RTA

Specificité: This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Porc

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-SISQVNHKPLRTA

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperatu

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Flotillin 1 (ABIN184756)

Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).

Détails sur Flotillin 1

Antigène: Flotillin 1 (FLOT1)

Autre désignation: FLOT1

Sujet: FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2

ID gène: 10211, 14251

NCBI Accession: NP_005794, NP_001305804