AIRE anticorps (C-Term)

N° du produit ABIN184780

Cet anticorps Chèvre Polyclonal détecte spécifiquement AIRE dans WB, ELISA, IF, IHC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour AIRE anticorps (C-Term) (ABIN184780)

Antigène: AIRE (Autoimmune Regulator (AIRE))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-AIRE anticorps

Épitope: C-Term

Fonction: AIRE

Séquence: QSMARPAAPF PS

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. R

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE

Sujet: AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop

ID gène: 326

NCBI Accession: NP_000374

Pathways: Chromatin Binding