ARIH2 anticorps (C-Term)

N° du produit ABIN184916

L’anticorps Chèvre Polyclonal anti-ARIH2 a été validé pour WB, ELISA et IF. Il convient pour détecter ARIH2 dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour ARIH2 anticorps (C-Term) (ABIN184916)

Antigène: ARIH2 (protein ariadne-2 homolog (ARIH2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ARIH2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ARIH2 anticorps

Épitope: C-Term

Fonction: ARIH2 / TRIAD1

Séquence: EQRRRTLLKD FHDT

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ARIH2 (ABIN184916)

Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." dans: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

Détails sur ARIH2

Antigène: ARIH2 (protein ariadne-2 homolog (ARIH2))

Autre désignation: ARIH2

Sujet: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2

ID gène: 10425, 23807

NCBI Accession: NP_006312