TRIM21 anticorps (C-Term)

N° du produit ABIN184965

Cet anticorps anti-TRIM21 est un anticorps Chèvre Polyclonal détectant TRIM21 dans WB, ELISA, IF et FACS. Adapté pour Humain.

Aperçu rapide pour TRIM21 anticorps (C-Term) (ABIN184965)

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM21 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-TRIM21 anticorps

Épitope: C-Term

Fonction: SSA1 / Ro52

Séquence: CPLNIGSQGS TDY

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antib

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TRIM21

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Autre désignation: TRIM21

Sujet: TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52 kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome ant

ID gène: 6737

NCBI Accession: NP_003132