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NUP50 anticorps (C-Term)

Cet anticorps anti-NUP50 est un anticorps Chèvre Polyclonal détectant NUP50 dans WB, IHC et ELISA. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.
N° du produit ABIN184968

Aperçu rapide pour NUP50 anticorps (C-Term) (ABIN184968)

Antigène

Voir toutes NUP50 Anticorps
NUP50 (Nucleoporin 50kDa (NUP50))

Reactivité

  • 44
  • 24
  • 14
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Humain

Hôte

  • 60
  • 4
Chèvre

Clonalité

  • 59
  • 5
Polyclonal

Conjugué

  • 38
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp NUP50 est non-conjugé

Application

  • 41
  • 16
  • 16
  • 13
  • 13
  • 10
  • 10
  • 9
  • 7
  • 3
  • 2
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité

Verified
  • Épitope

    • 15
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Fonction

    Npap60 / Nup50

    Séquence

    ELHKILLEKK DA

    Specificité

    This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).

     Réactivité croisée

    Boeuf (Vache), Humain, Souris, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogène

    Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.

    Isotype

    IgG
  • Indications d'application

    Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.

    Western Blot: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Minimize freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Shitashige, Satow, Honda, Ono, Hirohashi, Yamada: "Regulation of Wnt signaling by the nuclear pore complex." dans: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).

  • Antigène

    NUP50 (Nucleoporin 50kDa (NUP50))

    Autre désignation

    NUP50

    Sujet

    NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530

    ID gène

    10762, 18141

    NCBI Accession

    NP_009103, NP_705931

    Pathways

    Tube Formation
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