IFIH1 anticorps (N-Term)

N° du produit ABIN185002

Cet anticorps Chèvre Polyclonal détecte spécifiquement IFIH1 dans WB, ELISA, IHC et FACS. Il présente une réactivité envers Humain et Singe et a été mentionné dans 3+ publications.

Aperçu rapide pour IFIH1 anticorps (N-Term) (ABIN185002)

Antigène: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFIH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-IFIH1 anticorps

Épitope: N-Term

Fonction: MDA5 / IFIH1

Séquence: SNGYSTDENF RYL

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence SNGYSTDENFRYL-C, from the N Terminus of the protein sequence according to NP_071451.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10 μg/mL.

Western Blot: This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul,60(7):2193-200. PMID: 19565506.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-IFIH1 (ABIN185002)

Sato, Hoshino, Satoh, Fujita, Kawakami, Fujita, Kuwana et al.: "RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial ..." dans: Arthritis and rheumatism, Vol. 60, Issue 7, pp. 2193-200, (2009) (PubMed).

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." dans: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, (2008) (PubMed).

Bao, Sinha, Liu, Hong, Luxon, Garofalo, Casola: "Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis." dans: Virology, Vol. 374, Issue 1, pp. 114-27, (2008) (PubMed).

Détails sur IFIH1

Antigène: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Autre désignation: IFIH1

Sujet: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard

ID gène: 64135

NCBI Accession: NP_071451

Pathways: Activation of Innate immune Response