FOXA1 anticorps (C-Term)

N° du produit ABIN185021

L’anticorps Chèvre Polyclonal anti-FOXA1 a été validé pour WB, FACS, IF et ELISA. Il convient pour détecter FOXA1 dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour FOXA1 anticorps (C-Term) (ABIN185021)

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXA1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-FOXA1 anticorps

Épitope: C-Term

Fonction: FOXA1 / HNF3A

Séquence: GVYSRPVLNT S

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-FOXA1 (ABIN185021)

Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." dans: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).

Détails sur FOXA1

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Autre désignation: FOXA1

Sujet: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha

ID gène: 3169, 15375, 25098

NCBI Accession: NP_004487

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis