STK35 anticorps (C-Term)

N° du produit ABIN185178

Cet anticorps anti-STK35 est un anticorps Chèvre Polyclonal détectant STK35 dans WB, ELISA, IHC, IF et FACS. Adapté pour Humain, Souris et Porc. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour STK35 anticorps (C-Term) (ABIN185178)

Antigène: STK35 (Serine/threonine Kinase 35 (STK35))

Reactivité: Humain, Souris, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp STK35 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-STK35 anticorps

Épitope: C-Term

Fonction: Clik1 / STK35

Séquence: ELETRMDQVT CAA

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-STK35 (ABIN185178)

Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." dans: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

Détails sur STK35

Antigène: STK35 (Serine/threonine Kinase 35 (STK35))

Autre désignation: STK35

Sujet: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form

ID gène: 140901, 67333

NCBI Accession: NP_543026