TCP1 alpha/CCTA anticorps (C-Term)

N° du produit ABIN185185

Cet anticorps Chèvre Polyclonal détecte spécifiquement TCP1 alpha/CCTA dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour TCP1 alpha/CCTA anticorps (C-Term) (ABIN185185)

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TCP1 alpha/CCTA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-TCP1 alpha/CCTA anticorps

Épitope: C-Term

Fonction: TCP1

Séquence: SYEDAVHSGA LND

Specificité: This antibody is expected to recognise both reported isoforms.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TCP1 alpha/CCTA

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Autre désignation: TCP1

Sujet: TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1

ID gène: 6950

NCBI Accession: NP_110379, NP_001008897