ACTR1A anticorps (C-Term)

N° du produit ABIN185219

L’anticorps Chèvre Polyclonal anti-ACTR1A a été validé pour WB, ELISA et FACS. Il convient pour détecter ACTR1A dans des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour ACTR1A anticorps (C-Term) (ABIN185219)

Antigène: ACTR1A (Alpha Centractin (ACTR1A))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ACTR1A est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-ACTR1A anticorps

Épitope: C-Term

Fonction: ARP1 homolog A

Séquence: YEEDGARSIH RKT

Specificité: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 42-45 kDa band observed in lysates of cell lines A431 HEK293, HeLa, MCF7, NIH3T3, KNRK and MDCK, and in preliminary testing of Human and Mouse Adrenal gland and Mouse Stomach lysate (calculated MW of 42.6 kDa according to Human NP_005

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: A customer reported cytoplasmic staining on A431 cells at a primary ab concentration of 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ACTR1A

Antigène: ACTR1A (Alpha Centractin (ACTR1A))

Autre désignation: ACTR1A

Sujet: ACTR1A, ARP1, ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), actin-RPV, centractin alpha, ARP1, yeast homolog A, centrosome-associated actin homolog, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha), CTRN1, FLJ52695, F

ID gène: 10121, 54130

NCBI Accession: NP_005727

Pathways: M Phase