EHD2 anticorps (C-Term)

N° du produit ABIN185287

Cet anticorps Chèvre Polyclonal détecte spécifiquement EHD2 dans WB, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour EHD2 anticorps (C-Term) (ABIN185287)

Antigène: EHD2 (EH-Domain Containing 2 (EHD2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EHD2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-EHD2 anticorps

Épitope: C-Term

Fonction: EHD2

Séquence: CRLVPPSKRR HKGSA

Specificité: This antibody is expected to recognise EHD1 protein as well as EHD2.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur EHD2

Antigène: EHD2 (EH-Domain Containing 2 (EHD2))

Autre désignation: EHD2

Sujet: EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617

Poids moléculaire: 61.2kDa

ID gène: 30846

NCBI Accession: NP_055416

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development