Cytochrome B Reductase 1 anticorps (C-Term)

N° du produit ABIN185343

Cet anticorps anti-Cytochrome B Reductase 1 est un anticorps Chèvre Polyclonal détectant Cytochrome B Reductase 1 dans ELISA, WB et IHC. Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Cytochrome B Reductase 1 anticorps (C-Term) (ABIN185343)

Antigène: Cytochrome B Reductase 1 (CYBRD1)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Cytochrome B Reductase 1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-Cytochrome B Reductase 1 anticorps

Épitope: C-Term

Fonction: Cytochrome b reductase 1

Séquence: CRNLALDEAG QRSTM

Specificité: This antibody is expected to recognise isoform 1 (NP_079119.3) only.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Porc

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Small Intestine. Rec

Western Blot: Preliminary experiments gave bands at approx 35 kDa and a 26+28 kDa doublet in human colon lysate after 0.1 μg/mL antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfull

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Cytochrome B Reductase 1 (ABIN185343)

Dostalikova-Cimburova, Balusikova, Kratka, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease." dans: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1840-50, (2014) (PubMed).

Dostalikova-Cimburova, Kratka, Balusikova, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency." dans: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1816-26, (2012) (PubMed).

Balusikova, Neubauerova, Dostalikova-Cimburova, Horak, Kovar: "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess." dans: Molecular and cellular biochemistry, Vol. 321, Issue 1-2, pp. 123-33, (2009) (PubMed).

Détails sur Cytochrome B Reductase 1

Antigène: Cytochrome B Reductase 1 (CYBRD1)

Autre désignation: CYBRD1

Sujet: CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3

ID gène: 79901

NCBI Accession: NP_079119

Pathways: Transition Metal Ion Homeostasis