TPI1 anticorps (C-Term)

N° du produit ABIN185349

Cet anticorps anti-TPI1 est un anticorps Chèvre Polyclonal détectant TPI1 dans WB, ELISA, IHC et FACS. Adapté pour Humain, Souris, Rat et Chien. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour TPI1 anticorps (C-Term) (ABIN185349)

Antigène: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TPI1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-TPI1 anticorps

Épitope: C-Term

Fonction: Triosephosphate isomerase

Séquence: LKPEFVDIIN AKQ

Specificité: This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1, NP_001152759.1, NP_001244955.1.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7 kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-TPI1 (ABIN185349)

Honoré, Buus, Claësson: "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." dans: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).

Vorum, Østergaard, Rice, Honoré, Bek: "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study." dans: Proteome science, Vol. 5, pp. 5, (2007) (PubMed).

Détails sur TPI1

Antigène: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Autre désignation: TPI1

Sujet: TPI1, triosephosphate isomerase 1, TPI, MGC88108

ID gène: 7167, 21991, 24849

NCBI Accession: NP_000356, NP_001152759

Pathways: Cell RedoxHomeostasis