NPM1 anticorps (C-Term)

N° du produit ABIN185372

L’anticorps anti-NPM1 Polyclonal Chèvre est utilisé pour la détection de NPM1 dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, IF et ELISA.

Aperçu rapide pour NPM1 anticorps (C-Term) (ABIN185372)

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NPM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-NPM1 anticorps

Épitope: C-Term

Fonction: Nucleophosmin / NPM1

Séquence: QEAIQDLWQW RKSL

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6 kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed b

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NPM1

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Autre désignation: NPM1

Sujet: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1

ID gène: 4869

NCBI Accession: NP_001341935, NP_954654, NP_001341936, NP_001341939

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly