NPY5R anticorps (Internal Region)

N° du produit ABIN185409

Cet anticorps anti-NPY5R est un anticorps Chèvre Polyclonal détectant NPY5R dans WB, IHC, ELISA et IP. Adapté pour Humain et Hamster. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour NPY5R anticorps (Internal Region) (ABIN185409)

Antigène: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Reactivité: Humain, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NPY5R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-NPY5R anticorps

Épitope: Internal Region

Fonction: Neuropeptide Y receptor Y5

Séquence: GLSNKENRLE EN

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in W

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-NPY5R (ABIN185409)

Czarnecka, Kitlinska: "Cell Surface Protein Detection to Assess Receptor Internalization." dans: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).

Sheriff, Ali, Yahya, Haider, Balasubramaniam, Amlal: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." dans: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, (2010) (PubMed).

Détails sur NPY5R

Antigène: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Autre désignation: NPY5R

Sujet: Neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPYR5

Poids moléculaire: 50.7kDa

ID gène: 4889, 18168, 25340

NCBI Accession: NP_006165

Pathways: Feeding Behaviour