PPY anticorps (Internal Region)

N° du produit ABIN185420

L’anticorps Chèvre Polyclonal anti-PPY a été validé pour WB, IHC, ELISA, FACS et IF. Il convient pour détecter PPY dans des échantillons de Humain et Rat. Il y a 1 publication disponible.

Aperçu rapide pour PPY anticorps (Internal Region) (ABIN185420)

Antigène: PPY (Pancreatic Polypeptide (PPY))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PPY est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PPY anticorps

Épitope: Internal Region

Fonction: Pancreatic Polypeptide / PPY

Séquence: TRPRYGKRHK EDT

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 μg/mL. Paraffin embedded Human Pancreas and Intestine. Recommended co

Western Blot: This antibody has been successfully used in WB on Human, PMID: 25445712.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 27300574 and 30687234, and on Rat, PMID: 27472443.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PPY (ABIN185420)

Md Moin, Cory, Ong, Choi, Dhawan, Butler, Butler: "Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes." dans: Journal of the Endocrine Society, Vol. 1, Issue 5, pp. 385-395, (2017) (PubMed).

Détails sur PPY

Antigène: PPY (Pancreatic Polypeptide (PPY))

Autre désignation: PPY

Sujet: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y

ID gène: 5539

NCBI Accession: NP_002713

Pathways: Feeding Behaviour