Caveolin-1 anticorps (Internal Region)

N° du produit ABIN185421

Cet anticorps anti-Caveolin-1 est un anticorps Chèvre Polyclonal détectant Caveolin-1 dans WB, IHC, ELISA, IF et FACS. Adapté pour Humain.

Aperçu rapide pour Caveolin-1 anticorps (Internal Region) (ABIN185421)

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Caveolin-1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-Caveolin-1 anticorps

Épitope: Internal Region

Fonction: Caveolin 1

Séquence: DELSEKQVYD AH

Specificité: This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Réactivité croisée: Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5 kDa according to NP_001744.2). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at room temperature. Pre

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analy

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Caveolin-1

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Autre désignation: CAV1

Sujet: CAV1, caveolin 1, caveolae protein, 22 kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP08

ID gène: 857, 12389, 25404

NCBI Accession: NP_001744, NP_001166366

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals