PEPCK anticorps (Internal Region)

N° du produit ABIN185483

L’anticorps Chèvre Polyclonal anti-PEPCK a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter PEPCK dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour PEPCK anticorps (Internal Region) (ABIN185483)

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PEPCK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-PEPCK anticorps

Épitope: Internal Region

Fonction: PCK2 / PEPCK-M

Séquence: KPWKPGDKEP CAH

Specificité: No cross-reactivity with PCK1.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7 kDa according to Human NP_004554.3 and 70.5 kDa according to Mouse NP_083270.2). Recommended concentration: 1-3 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PEPCK (ABIN185483)

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." dans: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Détails sur PEPCK

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Autre désignation: PCK2

Sujet: PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase

ID gène: 5106

NCBI Accession: NP_004554

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis