TIM3 anticorps (Internal Region)

N° du produit ABIN185571

Cet anticorps anti-TIM3 est un anticorps Chèvre Polyclonal détectant TIM3 dans FACS, WB, ELISA et IF. Adapté pour Humain.

Aperçu rapide pour TIM3 anticorps (Internal Region) (ABIN185571)

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TIM3 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-TIM3 anticorps

Épitope: Internal Region

Fonction: TIM3 / HAVCR2

Séquence: KWYSHSKEKI QN

Réactivité croisée: Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KWYSHSKEKIQN, from the internal region of the protein sequence according to NP_116171.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 35 kDa band observed in lysates of cell lines A549 and HepG2 and in preliminary testing of Jurkat and U937 cell lysate (calculated MW of 33.4 kDa according to NP_116171.3). Recommended concentration:0.1-1 μg/mL. Primary incubation 1 hou

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended con

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TIM3

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Autre désignation: HAVCR2

Classe de substances: Virus

Sujet: HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3

ID gène: 84868

NCBI Accession: NP_116171

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints