EN2 anticorps (C-Term)

N° du produit ABIN185576

L’anticorps Chèvre Polyclonal anti-EN2 a été validé pour WB, ELISA et IF. Il convient pour détecter EN2 dans des échantillons de Humain et Souris.

Aperçu rapide pour EN2 anticorps (C-Term) (ABIN185576)

Antigène: EN2 (Engrailed Homeobox 2 (EN2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EN2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-EN2 anticorps

Épitope: C-Term

Fonction: Engrailed-2

Séquence: NHSTTAKEGK SDSE

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur EN2

Antigène: EN2 (Engrailed Homeobox 2 (EN2))

Autre désignation: EN2

Sujet: Engrailed-2, engrailed homolog 2 , EN2, AUTS1

ID gène: 2020

NCBI Accession: NP_001418